Table 1.

Biological samples collected by participant group

	ASD-Probands/ASD-Query	Parents	Siblings/Controls
Blooda
EDTA	6 ml	10 ml	6 ml
SST	5 ml	5 ml	5 ml
PAXgeneb	2.5 ml	2.5 ml	2.5 ml
Stoolc	2 × 1 teaspoon	–	2 × 1 teaspoon
Urinec	~  20 ml	–	~  20 ml
Hairc	~ 10 strands	–	~ 10 strands

^aSaliva collection was attempted where a blood sample collection was unsuccessful; ^bPAXgene samples (for RNA) collected on a sub-set of participants; ^cStool, urine, and hair collected on a sub-set of children