Fig. 2.

Course of biomass concentrations during the anaerobic phase of two-stage cultivation. Shown are biomass measurements for the wild tpye MG1655 (black), KBM151101 (red), SB2 (green) and KBM1673 (blue). Biomass concentration is plotted on a logarythmically scaled axis. At the start of the anaerobic phase, all strains were inoculated to the same cell density (OD₄₂₀ ~ 2). While MG1655 shows quick anaerobic growth, growth of all mutant strains is strongly impaired. The data shown are derived from one exemplary experiment. At least three repeats have been carried out for all strains amd average data concerning growth rates are given in Table 3