Table 4.

Comparison of predicted and observed succinate yields for the mutant strains

	Observed Yxs [$\frac{\mathit{gDW}}{mmolglucose}$]	Observed Yps [$\frac{molsuccinate}{molglucose}$]	Strain adapted theoretical yield [$\frac{molsuccinate}{molglucose}$]	Percentage of adapted optimum [%]
KBM151101	0	0.10	1	10
SB2	0.010	1.24	1.59	78
SB2/pPckA	0.006	1.42	1.64	86
SB2/pPpc	0	1.08	1.71	63
KBM1673	0	0.83	1.71	48
KBM1673/pPckA	0.025	0.92	1.4	65
KBM1673/pPpc	0	1.05	1.71	61

Yxs: observed biomass yield (as shown in Table 3); Yps: observed succinate yield (as in Table 3); adapted theoretical yield given by the maximal yield predicted by FBA when constrained by observed biomass yield and genetic background; percentage of adapted optimum calculated by dividing the observed succinate yield by the strain adapted optimal yield