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. 2001 Nov 20;98(24):13802–13807. doi: 10.1073/pnas.241508098

Figure 1.

Figure 1

CAD gene amplification in PALA-resistant derivatives of MT1 and HCT116. FISH was performed as described in Experimental Procedures. The two micrographs (Left) illustrate results with PALA-sensitive MSH6−/− MT1 cells (Upper) and a PALA-resistant derivative (Lower). Similar results with MLH1−/− HCT116 cells are shown Right. The pink fluorophore corresponds to a probe for chromosome 2 alpha satellite DNA, while the green fluorophore is the CAD gene probe. Note that CAD amplification is also evident in interphase nuclei, which were present in the same field with the mitotic chromosome spreads in three of the cases shown.