Table 2.
Name | Primer sequence | Purpose |
---|---|---|
P1 | TTACCTTGTTCCGTTGCTTT | Determine insert position |
P2 | CTGCAACTTCCTGATTGTGC | Determine insert position |
P3 | GCACAATCAGGAAGTTGCAG | Determine insert position |
P4 | CATGGACAGCACACCTTTGT | Determine insert position |
P5 | AGCAGTTTTGCACCCTATGT | Determine insert position |
P6 | CCATTTGTCCGTACTCTTGC | Determine insert position |
D1-R2-2 | CCAGCCTTTCAAACGAACCA | Tail-PCR |
D1-R2 | AGCAGTTTTGCACCCTATGT | Tail-PCR |
D1-EXON6-R1 | CCTTCGTTATGTAGACTGCGT | Tail-PCR |
CT-R2 | ACACAGTACCGACGTCTTGT | Osr4 expression |
T-F2 | GCCGAGGAGATCACTAAGCA | Osr4 expression |
CT-R3 | TCCTTCTACCTGGGGATCGA | Osr4 expression |
T-F1 | AAGCCATGTCCTCCCAAAGA | Osr4 expression |
D1-cDNA-F1 | ACGTGCTTCCTGGAAAGAGA | D1 cDNA |
D1-cDNA-R2 | AGACCTGAACAGCCCACAGT | D1 cDNA |
RT-F | TGCTGCCATTAGCGAATATG | D1 gene expression |
RT-R | GGCTTGCTGCTCTGGAAGTA | D1 gene expression |