Table 2.

Characteristics of the 16 validated polymorphic microsatellite loci developed for Taxus florinii.

Locus	GenBank accession no.	Repeat motif	Primer sequence (5′–3′)	Ta (°C)	Size (bp)
gr907	KY704069	(CTT)6–7	F:CATTGCGCCTCTTTGGAGTC	58	113–116
			R:TGGCAGGCAGAATCAAAGGT
gr1114	KY704070	(TG)7–10	F:AGACCCATCCAATATTTATAAAATGGT	58	106–112
			R:AGAGACAACTTGAAAAGACCAGA
gr1177	KY704071	(AT)8–9	F:AGACCACACTTGCCCTTCAG	58	109–111
			R:AGCCAATGTATGCTAGGGAGTG
gr1258	KY704072	(TA)9–10	F:GACCCCACATTGTTTCTTCAAG	58	115–117
			R:ACAACCTCTCAAAAACAACACAA
gr1439	KY704073	(TC)7–8	F:TACCAATCACCCACCGTTCG	58	123–125
			R:GCCTCTTGCCATGACCGTTT
gr2591	KY704074	(TC)6–7	F:GCCCTTAACGACGGAGTTGA	58	111–113
			R:CTCCTCTCTTGATAGCCGCG
gr2662	KY704075	(AT)8–9	F:GTCCTATGTACCACTAAGCACA	58	137–139
			R:CGTTGTCTCAAGAATTTAATACATTCC
gr2680	KY704076	(TA)8–9	F:AGTCAAAATGAGACCCATGACCA	58	126–128
			R:CGAGTTTAAGATGAATTTATAGCAAGC
gr4557	KY704077	(CATTGA)9–11	F:CATCATGAAGGTCAGCCCGT	58	106–118
			R:AGATACCCTAATTAAGAGAGAAGGTG
gr4772	KY704078	(GAGAGG)5–6	F:TACGGGGCGGTTAGGGATTA	58	108–114
			R:TGCTGTTCCAACAACCTCCT
gr6458	KY704079	(CTC)9–11	F:GTGAGCGCGGACTTTTGC	58	119–125
			R:GCCACTACCGAACGCTTAGT
gr1031	KY704080	(GAGAA)6–8	F:CATGGTTCCCCGTCTGGAAA	60	108–118
			R:TGTGGGGTGGGGAATGATTG
gr5502	KY704081	(AGATA)5–6	F:AGCGGTGCAGAGTTTGATGA	60	104–109
			R:TTGTGGTCATTCGTTGGACA
gr2164	KY704082	(TTAG)5–6	F:GTCCACAAGAAACCTCACCCT	60	130–134
			R:ATAACAAACTCTTGGCGGCG
gr1415	KY704083	(CTG)6–7	F:ACCACCAGCTGCATCATGAA	60	127–130
			R:GGATTGGGAATGGCAATGGC
gr5371	KY704084	(AGA)17–19	F:CCAAACGGGTTGCCTGAGAT	60	136–142
			R:CCCCCACACACCATCCTTTT