Table 1.
Characteristics of the eight primers tested for E. phyllopogon genotyping: locus name, forward (F) and reverse (R) primer sequences, motif, annealing temperature (Tm), fluorescent dye used (Fl. dye), allele size range (ASR), number of alleles amplified per sample, and number of alleles amplified among the plants of four populations sampled (Allele. total).
| Marker | Sequence | Motif | ASR (bp) | Fl. dye | Tm (°C) | No. of alleles per sample |
Allele. total | ||
|---|---|---|---|---|---|---|---|---|---|
| Min. | Mean | Max. | |||||||
| EG _1 | F: GCTCCTGAACTGTGTACATTCTTGC | TG | 123–153 | TAM | 49 | 0 | 0.7 | 2 | 5 |
| R: TCGATTCACCCTTCAGCTTCTC | |||||||||
| EG_2 | F: CATCGGATTCAGATTGAAAGGG | TA | 131–159 | FAM | 51.5 | 1 | 1.7 | 3 | 7 |
| R: GGTCGTAGGTCTATAGTCCGTAGAGTCA | |||||||||
| EG_301 | F: GCGTCGTCAAGTCGTTCTTCTA | AT | 147–173 | TAM | 57 | 0 | 2.4 | 3 | 8 |
| R: TGTATTCAGCTGTCGTGCATGT | |||||||||
| EG_302 | F: ATTCGAACACCCATCAACCAAC | ATTT | 133–293 | FAM | 57 | 1 | 2.8 | 5 | 12 |
| R: GAAACAGAAGGGAGGTGTGCTG | |||||||||
| EG_305 | F: AGCCGTTCCTCTAGTCGGATTTCT | AT | 100–162 | ROX | 57 | 3 | 4.1 | 6 | 14 |
| R: TATTCAGCTGCCGTGCATGTAGTA | |||||||||
| EG_306 | F: TAAAACAAAACGACCGGCGTAA | CT | 146–167 | HEX | 57 | 1 | 1.25 | 2 | 7 |
| R: TCAATCATTTCAGCCTTCGGAT | |||||||||
| EG_307 | F: AACATTGTCATCACAAATATCATCATCA | ATC | 108–134 | TAM | 57 | 2 | 3.5 | 5 | 8 |
| R: AATCAAGGAAGCCCCTTCACTC | |||||||||
| EG_320 | F: CAACTCATAAGACAATTCAAAGGGTTT | TA | 136–153 | FAM | 57 | 2 | 3.0 | 4 | 5 |
| R: GCATCATTTAAGCATCAAAATGACA | |||||||||
FAM: 6-carboxyfluorescein, HEX: hexachloro-fluoresceine, ROX: carboxy-X-rhodamine, and TAM: 5-TAMRA (5-Carboxytetramethylrhodamine).