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. 2001 Nov 20;98(24):13808–13813. doi: 10.1073/pnas.241304198

Figure 3.

Figure 3

Immunoprecipitation of the PRCC and MAD2B proteins. Lanes 1–8: immunoprecipations (IPs) performed on single transfectants by using an anti-GFP/YFP antibody (lanes 1 and 5) to precipitate PRCC and an anti-MAD2B antibody (lanes 2 and 6) to precipitate MAD2B. Lanes 3, 7, and 11 contain IP products obtained with preimmune sera instead of specific antibodies. Lanes 4, 8, and 12 contain 0, 5 μl of the total protein samples used for the IPs. Lanes 9–12 contain IP products of cotransfected cells by using anti-GFP/YFP (lane 9) or anti-MAD2B antibodies (lane 10), showing that MAD2B coimmunoprecipates with PRCC and vice versa (arrows). The middle section (lanes 9, 10) is a partial lighter exposure of the right section. Size markers (Bio-Rad) are indicated (Left and Right).