Table 2.
Primers used for quantitative real-time PCR.
| Primer | Sequence (5′ to 3′) | Size, bp | Literature cited |
|---|---|---|---|
| Bacteria-F | CGGCAACGAGCGCAACCC | 146 | Denman and McSweeney (2006) |
| Bacteria-R | CCATTGTAGCACGTGTGTAGCC | ||
| Fungi-F | GAGGAAGTAAAAGTCGTAACAAGGTTTC | 120 | Denman and McSweeney (2006) |
| Fungi-R | CAAATTCACAAAGGGTAGGATGATT | ||
| Protozoa-F | GCTTTCGWTGGTAGTGTATT | 223 | Sylvester et al. (2004) |
| Protozoa-R | CTTGCCCTCYAATCGTWCT | ||
| Methanogen-F | TTCGGTGGATCDCARAGRGC | 140 | Denman et al. (2007) |
| Methanogen-R | GBARGTCGWAWCCGTAGAATCC | ||
| Fibrobacter succinogenes-F | GTTCGGAATTACTGGGCGTAAA | 121 | Denman and McSweeney (2006) |
| Fibrobacter succinogenes-R | CGCCTGCCCCTGAACTATC | ||
| Selenomonas ruminantium-F | CAATAAGCATTCCGCCTGGG | 138 | Stevenson and Weimer (2007) |
| Selenomonas ruminantium-R | TTCACTCAATGTCAAGCCCTGG | ||
| Ruminococcus albus-F | CCCTAAAAGCAGTCTTAGTTCG | 176 | Koike et al. (2001) |
| Ruminococcus albus-R | CCTCCTTGCGGTTAGAACA | ||
| Prevotella ruminicola-F | GAAAGTCGGATTAATGCTCTATGTTG | 74 | Stevenson and Weimer (2007) |
| Prevotella ruminicola-R | CATCCTATAGCGGTAAACCTTTGG |
F = forward; R = reversed.