Table 1.

Ingredient and nutrient composition of the experimental layer diet (DM basis).¹

Item	Control	T1 and T2	T3 and T4
Ingredient, %
Maize	57.75	57.75	57.95
Soybean meal	23	23	23
De oiled rice bran	10	10	10
Limestone	7.61	8.11	8.41
Salt (NaCl)	0.30	0.30	0.30
CaHPO4	1.0	0.5	–
DL-methionine	0.09	0.09	0.09
Trace mineral and vitamin premix2	0.25	0.25	0.25
Nutrient composition, %
ME,3 kcal/kg	2,614	2,614	2,620
CP	17.1	17.1	17.1
Ca	3.53	3.50	3.42
Total P	0.632	0.552	0.472
Phytate P	0.308	0.308	0.308
Available P3	0.32	0.24	0.16
Lysine	0.74	0.74	0.74
Methionine	0.34	0.34	0.34
Threonine	0.62	0.62	0.62

¹Control = 0.33% non-phytate phosphorus (NPP); T1 = 0.24% NPP + 250 FTU/kg laboratory produced phytase; T2 = 0.24% NPP + 250 FTU/kg commercial phytase; T3 = 0.16% NPP + 500 FTU/kg laboratory produced phytase; T4 = 0.16% NPP + 500 FTU/kg commercial phytase.

²Trace mineral premix, 1 g/kg; vitamin premix, 1 g/kg and choline, 0.5 g/kg. Trace mineral premix supplied mg/kg diet: Mg, 300; Mn, 55; I, 0.4; Fe, 56; Zn, 30; Cu, 4. Vitamin premix supplied per kg diet: vitamin A, 8250 IU; vitamin D₃, 30 mg; vitamin K, 1 mg; vitamin E, 40 IU; vitamin B₁, 2 mg; vitamin B₂, 4 mg; vitamin B₁₂, 0.01 mg; niacin, 60 mg; pantothenic acid, 10 mg.

³Calculated values.