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. 2017 Sep 4;4(1):90–99. doi: 10.1016/j.aninu.2017.08.008

Table 3.

Effect of alfalfa seedling inoculation and co-inoculation with root growth promoting microorganisms on alfalfa hay Fourier transformed infrared spectroscopy (FTIR) molecular structures.

Item1 Treatments2
C Pi Gi + Pi Sm + Pi SEM P-value
FTIR molecular structures peak height
TCHO 0.02837 0.02581 0.03078 0.03057 0.007246 0.95
SCHO 0.01193 0.01304 0.01488 0.01495 0.003919 0.93
Proteins (amide 1 + amide 2) 0.01449 0.01498 0.01856 0.01899 0.004478 0.84
Lipids 0.00743 0.00852 0.00920 0.00932 0.002659 0.95
FTIR molecular peak height ratios
Proteins: SCHO 1.2155 1.1414 1.2893 1.3452 0.06881 0.22
Proteins:TCHO 0.5124b 0.575a 0.5938a 0.6247a 0.02068 0.01
Protein:lipid 1.9586 1.8315 2.1191 2.1750 0.10,450 0.13
SCHO:TCHO 0.4262 0.5044 0.4649 0.4713 0.03066 0.38
Lipids:TCHO 0.2626 0.3118 0.2825 0.2912 0.02074 0.30

SEM = standard error of the mean; SCHO = structural carbohydrate; TCHO = total carbohydrate.

a, b Within a row, means without a common superscript differ (P < 0.05).

1

TCHO, 852 to 1,186/cm; SCHO, 1,188.9 to 1,482.9/cm; Proteins (amide 1 + amide 2), 1,483 to 1,708/cm; lipids (CH2 and CH3, symmetric and antisymmetric functional groups) 2,770 to 3,000/cm; proteins: SCHO, ratio of proteins (amide 1 + amide 2) to SCHO; proteins: TCHO, ratio of proteins (amide 1 + amide 2) to TCHO; proteins: lipids, ratio of proteins (amide 1 + amide 2) to lipids; SCHO: TCHO, ratio of SCHO to TCHO.

2

C, control; Pi, seedlings inoculated with P. indica; Gi + Pi, co-inoculation of seedlings with P. indica and G. intraradices; Sm + Pi, co-inoculation of seedlings with P. indica and S. meliloti.