Figure 1.

Adipose induction of STS in obese mice, and creation of Tg mice expressing STS in the adipose tissue. (A) The mRNA expression of mouse Sts in the epididymal WAT of chow-fed wild-type (Wt) mice, HFD-fed Wt mice, and chow-fed ob/ob mice. (B) The schematic representation of the Tet-off STS Tg system. (C) The expression of STS protein in the epi-WAT and brown adipose tissue (BAT) of the Wt and Tg mice was measured by Western blotting. (D) The adipose STS enzymatic activity was determined by estrone sulfate conversion assay and was normalized against protein concentrations. (E) The adipose expression of the STS transgene in female and male Tg mice was measured by real-time PCR. n = 4 mice per group. * or #, P < 0.05; ** or ##, P < 0.01; ***P < 0.001, compared with (A) chow of the sex, or (D) or compared with the Wt. Pmin, minimal human cytomegalovirus promoter; PolyA, polyacrylamide; SV40 polyA, simian virus 40 polyadenylation signal.