Figure 3.

Adipose overexpression of STS decreases lipolysis and adipogenesis and aggravates HFD-induced adipose and systemic inflammation in males. Mice are the same as described in Fig. 2. The epi-WAT expression of genes responsible for (A) lipolysis, (B) glucose uptake and energy expenditure, and (C) lipogenesis and adipogenesis was measured by real-time PCR. (D) The protein level of PPARγ and ERK1/2 was measured by Western blotting. (E) Epi-WAT expression of adiponectin. (F) Immunostaining of Cd68. Shown on the right is the quantification. The (G) serum level of IL-6 and (H) adipose expression on proinflammatory genes and macrophage marker genes. Results are expressed as mean ± SD; n = 4 mice per group. *P < 0.05; **P < 0.01; ***P < 0.005, compared with the Wt.