Figure 7.

The metabolic benefit in female Tg mice is estrogen dependent. Mice are the same as described in Fig. 5. Shown are adipose expression of (A) estrogen-responsive genes and (B) androgen-responsive genes as measured by real-time PCR. Female mice were ovariectomized before being fed with HFD for 20 weeks. Shown are (C) adipose expression of estrogen-responsive genes, (D) body weight and body composition analysis, (E) oxygen consumption, (F) GTT and ITT, (G) immunostaining of CD68, (H) adipose expression of proinflammatory genes and macrophage marker genes, and (I) genes involved in adipogenesis and lipogenesis. Results are expressed as mean ± SD; n = 4 mice per group. *P < 0.05; **P < 0.01, compared with the Wt.