Table 1. Absolute copy numbers in RNA of uterus epithelium, granulosa and cumulus cells at 2 h (A, Group 1, n = 9 gilts) and at 17 h (B, Group 2: n = 7 gilts) after single uterine horn infusion of seminal plasma (SP) and contralateral infusion of PBS (Control = C); Means ± SEM.
A) 2 h | PTGS2 | IL6 | PPARG1 | PTX3 | TNFA | TNFAIP6 | UBB | |||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localisation | SP | C | SP | C | SP | C | SP | C | SP | C | SP | C | SP | C |
Uterine horn* | 16256 ± 4787 | 16995 ± 2519 | 170 ± 24 | 184 ± 18 | < 100 ± | < 100 | < 100 | < 100 | 1240 ± 360 | 893 ± 329 | 526 ± 108 | 455 ± 95 | 106771 ± 15108 | 80480 ± 5664 |
Utero-tubal Junction* | 4128 ± 2609 | 10271 ± 3299 | 185 ± 60 | 211 ± 22 | < 100 | < 100 | < 100 | < 100 | 315 ± 80 | 452 ± 147 | 191 ± 29 | 296 ± 56 | 93193 ± 11831 | 93636 ± 7483 |
Granulosa cells** | 45596 ± 15201 | 52563 ±21808 | < 100 | < 100 | 740 ± 261 | 720 ± 207 | 75914 ± 23078 | 100239 ± 33035 | < 100 | < 100 | 14417 ± 8205 | 9164 ± 6873 | 80118 ± 11211 | 72933 ± 6800 |
Cumulus cells*** | 4449 ± 1774 | 4662 ± 2062 | < 100 | < 100 | 143 ± 35 | 183 ± 46 | 10519 ± 5344 | 7617 ± 2297 | < 100 | < 100 | 7679 ± 6209 | 3513 ± 1882 | 20089 ± 3650 | 20545 ± 4669 |
B) 17 h | ||||||||||||||
Uterine horn* | 5633a ± 1375 | 11880b ± 2842 | < 100 | < 100 | < 100 | < 100 | < 100 | < 100 | 1006 ± 324 | 1409 ± 221 | 472 ± 136 | 677 ± 186 | 49941 ± 13344 | 53543 ± 8916 |
Utero-tubal Junction* | 2663 ± 699 | 6070 ± 2988 | < 100 | < 100 | < 100 | < 100 | < 100 | < 100 | 323 ± 90 | 519 ±172 | 323 ± 90 | 519 ± 172 | 57645 ± 15226 | 37777 ± 9352 |
Granulosa cells** | 56414 ±12794 | 57262 ±13781 | < 100 | < 100 | 422 ± 84 | 408 ± 50 | 174506 ±14924 | 182682 ±32869 | < 100 | < 100 | < 100 | < 100 | 82572 ±15435 | 68837 ± 11347 |
Cumulus cells*** | 16036 ± 3937 | 14778 ± 3805 | < 100 | < 100 | < 100 | < 100 | 25511 ± 4949 | 33045 ± 6358 | < 100 | < 100 | 1762 ± 674 | 624 ± 143 | 18388 ± 2690 | 17288 ± 2342 |
a, b: Significantly different (p< 0.05)
* 15 ng total RNA;
** 17 ng total RNA;
*** 4.5 ng total RNA