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. 2018 Aug 28;13(8):e0202654. doi: 10.1371/journal.pone.0202654

Table 1. Absolute copy numbers in RNA of uterus epithelium, granulosa and cumulus cells at 2 h (A, Group 1, n = 9 gilts) and at 17 h (B, Group 2: n = 7 gilts) after single uterine horn infusion of seminal plasma (SP) and contralateral infusion of PBS (Control = C); Means ± SEM.

A) 2 h PTGS2 IL6 PPARG1 PTX3 TNFA TNFAIP6 UBB
Localisation SP C SP C SP C SP C SP C SP C SP C
Uterine horn* 16256 ± 4787 16995 ± 2519 170 ± 24 184 ± 18 < 100 ± < 100 < 100 < 100 1240 ± 360 893 ± 329 526 ± 108 455 ± 95 106771 ± 15108 80480 ± 5664
Utero-tubal Junction* 4128 ± 2609 10271 ± 3299 185 ± 60 211 ± 22 < 100 < 100 < 100 < 100 315 ± 80 452 ± 147 191 ± 29 296 ± 56 93193 ± 11831 93636 ± 7483
Granulosa cells** 45596 ± 15201 52563 ±21808 < 100 < 100 740 ± 261 720 ± 207 75914 ± 23078 100239 ± 33035 < 100 < 100 14417 ± 8205 9164 ± 6873 80118 ± 11211 72933 ± 6800
Cumulus cells*** 4449 ± 1774 4662 ± 2062 < 100 < 100 143 ± 35 183 ± 46 10519 ± 5344 7617 ± 2297 < 100 < 100 7679 ± 6209 3513 ± 1882 20089 ± 3650 20545 ± 4669
B) 17 h
Uterine horn* 5633a ± 1375 11880b ± 2842 < 100 < 100 < 100 < 100 < 100 < 100 1006 ± 324 1409 ± 221 472 ± 136 677 ± 186 49941 ± 13344 53543 ± 8916
Utero-tubal Junction* 2663 ± 699 6070 ± 2988 < 100 < 100 < 100 < 100 < 100 < 100 323 ± 90 519 ±172 323 ± 90 519 ± 172 57645 ± 15226 37777 ± 9352
Granulosa cells** 56414 ±12794 57262 ±13781 < 100 < 100 422 ± 84 408 ± 50 174506 ±14924 182682 ±32869 < 100 < 100 < 100 < 100 82572 ±15435 68837 ± 11347
Cumulus cells*** 16036 ± 3937 14778 ± 3805 < 100 < 100 < 100 < 100 25511 ± 4949 33045 ± 6358 < 100 < 100 1762 ± 674 624 ± 143 18388 ± 2690 17288 ± 2342

a, b: Significantly different (p< 0.05)

* 15 ng total RNA;

** 17 ng total RNA;

*** 4.5 ng total RNA