Fig. 3.

Absence of AT2R gene expression in DRG sensory neurons and microglia/MΦs without or with nerve injury/neuropathy. (A) The Agtr2 gene (coding for AT2R) is not expressed in neurons and nonneuronal cells in mouse DRG, as verified by lack of GFP signal in DRG sections from Agtr2^GFP reporter mice, in which the Agtr2 promoter drives GFP expression. DRG sections are stained with CGRP and NF200 antibodies to mark peptidergic and myelinated sensory neurons. (Scale bars, 50 μm.) (B) A subset of sciatic nerve fibers of Agtr2^GFP mice are GFP⁺ (green). Such fibers are CGRP⁻ (red; Upper), and NF200⁺ (red; Lower) DAPI: blue. (Scale bars, 200 µm.) (C) Seven days following SNI surgery, there is an appreciable increase in Iba1⁺ cells (red; Center) in ipsilateral vs. contralateral DRG, wherein GFP signal (green; Left) remains negligible. DAPI: blue. (Scale bars, 50 μm.)