Fig. 5.

Up-regulation of lamin A/C is associated with polycomb or Casz1 loss of function. (A–C) Airyscan confocal images of retinal explants transfected with control constructs or shRNA constructs targeting Ring1 or Rnf2. Arrowheads mark heterochromatin contacting the nuclear lamina specifically in transfected cells. (D–F) Airyscan confocal analysis of lamin A/C or Lbr expression in Casz1-cKO retinas generated using the α-Pax6::Cre driver. The central retina does not express Cre during development and serves as an internal control (D). (E and F) Arrows mark lamin A/C up-regulation in basally located rod nuclei. The arrowhead in F marks an apically localized cone nucleus, which normally expresses lamin A/C. (Magnification: A–C, 6,300×; D and E, 945×; F, 3,150×.)