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. 2018 Aug 6;115(34):E7978–E7986. doi: 10.1073/pnas.1807918115

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Copyright © 2018 the Author(s). Published by PNAS.

This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND).

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Fig. 2. — Cysteine-reactivity profiling of the oocyte-to-embryo transition. (A) Pie charts showing the distribution of cysteine reactivity of the Drosophila stage-14 enriched ovary and 0- to 1-h embryo proteome. (B) Correlation of proteomic cysteine reactivity quantified in stage-14 enriched ovaries and 0- to 1-h embryos. The determined R_10:1 values of the cysteines present in both ovary and embryo samples are plotted against each other for comparison. Cysteines with an R^ova_10:1/R^emb_10:1 ratio >2 are in blue; cysteines with an R^ova_10:1/R^emb_10:1 ratio <0.5 are in red. Heavy/light (100 μM/10 μM) ratios were calculated from at least three biological replicates, with two technical runs for each.