Table 5.

Ethidium fluorescence plateau and efflux rates (see Materials and Methods)

Peptide	Ft→∞/F0*	EmrE efflux rate, $s^{-1}$†	Rel. EmrE efflux rate, %‡	Inhibition, %§
No peptide	0.16 ($\pm$0.02)	2.47 $\times \hspace{0.17em}{\mathrm{10}}^{-3}$ ($\pm$7.3 $\times \hspace{0.17em}{\mathrm{10}}^{-5}$)	100.0	0
A87-I94	0.67 ($\pm$0.05)	1.03 $\times \hspace{0.17em}{\mathrm{10}}^{-3}$ ($\pm$8.3 $\times \hspace{0.17em}{\mathrm{10}}^{-5}$)	42	58
I88-C95	0.16 ($\pm$0.04)	2.66 $\times \hspace{0.17em}{\mathrm{10}}^{-3}$ ($\pm$1.2 $\times \hspace{0.17em}{\mathrm{10}}^{-4}$)	108	0
I89-A96	0.38 ($\pm$0.05)	1.98 $\times \hspace{0.17em}{\mathrm{10}}^{-3}$ ($\pm$1.0 $\times \hspace{0.17em}{\mathrm{10}}^{-5}$)	80	20
M92-L99	0.77 ($\pm$0.07)	0.60 $\times \hspace{0.17em}{\mathrm{10}}^{-3}$ ($\pm$5.0 $\times \hspace{0.17em}{\mathrm{10}}^{-5}$)	24	76

^*Final normalized ethidium fluorescence plateau.

^†EtBr efflux rates are corrected for the passive background influx of ethidium from the extracellular medium (see Materials and Methods).

^‡Relative to cells without peptide.

^§Computed as 100% – EmrE efflux rate (%). Parentheses report 95% confidence limits.