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The S5N-term region helps keep spHCN channels closed. (A) Sequence alignment and homology model with S4C-term and S5N-term residues in blue. (B–D) ZD7288-subtracted currents and GV curves: (B) split spHCN(350:360), (C) split spHCN(350:363), and (D) split spHCN(350:367).
