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. 2018 Aug 7;9(61):31904–31914. doi: 10.18632/oncotarget.25881

Figure 5. Experimental design for analysis of influence of long-term storage on cfDNA and plasma.

Figure 5

Blood samples were obtained from 22 patients with advanced lung cancer harboring EGFR T790M mutation detected 7 years previously. The EGFR T790M test was performed with MBP-QP immediately after blood collection and DNA extraction (i), and again 7 years after storage of cfDNA at −20° C (ii). Five plasma samples among the 22 patients were stored at −80° C for 7 years. cfDNA was isolated from these five plasma samples with two different isolation methods: silica membrane spin column (iii) and cellulose magnetic beads system (iv).