Figure 4. The effect of loss of CD38 on the properties of early stage and size-matched tumors.

Paraffin sections prepared from ∼140 mm³ tumors from WT and Cd38^−/− B16F10-injected mice were analyzed for the amount of mitotic cells, thickness of the peritumoral region, amount of CAFs and density of blood vessels. The representative images of these analyses are shown in Supplementary Figure 1. (A) Quantitation of the number of mitotic cells. The data presented are expressed as the mean ± SEM (bars) of the number of mitotic cells in mm² (n = 8 WT and 9 Cd38^‒/‒ mice). n.s = not significant, Student’s t test. (B) Quantification of the amount of BrdU staining. The data presented are expressed as the mean ± SEM (bars) of the average number of BrdU-positive cells per field. (n = 8 WT and 9 Cd38^‒/‒ mice). n.s = not significant, Student’s t test. (C) Quantification of the peritumoral area. The results are expressed as the average area of peritumoral capsule in each tumor section (*p = 0.03; Student’s t test, n = 8 WT and 9 Cd38^‒/‒ mice). (D) Quantitation of the density of α-SMA staining. The results are expressed as the average number of α-SMA in the counted regions. (*p = 0.02; Student’s t test, n = 8 WT and 9 Cd38^‒/‒ mice). (E) Quantitation of the density of CD34 staining in the tumor area. The results are expressed as the percentage of CD34-positive area analyzed in each tumor section (*p = 0.04; Student’s t test, n = 8 mice from each group).