(A) Representative results of gelatin zymography analysis of media collected from U87MG cells incubated with 100 nM of the inhibitors for 48 h and resolved by SDS-PAGE. The bands in the top panel reflect inactive pro-MMP2, while the boxed region in the bottom panel contains bands that reflect active MMP2. The bottom panel in fact corresponds to the same gel used to generate the top panel, albeit after enhanced exposure and with increased sensitivity of detection. Specifically, to generate the lower panel, only the upper bright bands in gel were covered and a picture of the lower bands with 6-fold increased exposure and a 2-fold increase in sensitivity was taken. In addition, the contrast was increased. (B) Quantification of band intensity from the gel containing active MMP2, normalized to the intensities of bands from gels of control (untreated) cells. The experiment was repeated three times; means and standard error are given. *P < 0.05, **P < 0.01, as determined by t tests comparing the indicated condition versus the untreated control.