Table 1.
Antibodies used in study.
| Name | Isotype | Species | Dilution | Immunogen | Binding region | Source |
|---|---|---|---|---|---|---|
| ECAD Antibodies | ||||||
| 7D6 (Lcam) | mouse IgG1 |
Chicken | 1:10 | Ft1 fraqment | N-terminus (Extracellular) |
DSHB |
| 610181 (Anti-E- cadherin) | mouse IgG2a |
Human | 1:1000 | AA 735–883 | C-terminus (Cytoplasmic) |
BD Transduction Laboratories |
| 07–697 (Anti-E- Cadherin) | rabbit IgG |
Human | 1:1000 | AA 859–874 | C-terminus (Cytoplasmic) |
EMD Millipore |
| 8C2 (cadherin, E) | mouse IgG1 |
Xenopus | 1:10 | Tryptic fraqment | N-terminus (Extracellular) |
DSHB |
| NCAD Antibodies | ||||||
| MNCD2 (Anti-N- Cadherin) | rat IgG2a | Mouse | 1:10 | AA 308–597 | N-terminus (Extracellular) |
DSHB |
| 6B3 (cadherin, N) | mouse IgG1 |
Chicken | 1:10 | Ncad-α-catenin complex |
N-terminus (Extracellular) |
DSHB |
| ab18203 (Anti-N- cadherin) | rabbit IgG |
Human | 1:1000 | AA 800–900 | C-terminus (Cytoplasmic) |
Abcam |
| CAD7 Antibodies | ||||||
| CCD7–1 (cadherin-7) |
mouse IgG1 |
Chicken | 1:10 | N-term 597 AA and the human immunoglobulin Fc region. |
N-terminus (Extracellular) |
DSHB |
| ab71412 (Anti- Cadherin 7 antibody - N- terminal) |
rabbit IgG |
Human | 1:50 | N-terminal region of human Cadherin 7 conjugated to KLH |
N-terminus (Extracellular) |
Abcam |
| CAD6B Antibodies | ||||||
| ab64917 (Anti-K) | rabbit IgG |
Human | 1:50 | C-terminal region of human K Cadherin | C-terminus (Cytoplasmic) |
Abcam |
| CCD6B-1 (cadherin-6B) |
mouse IgG1 |
Chicken | 1:10 | N-term 605 AA and the human immunoglobulin Fc region. |
N-terminus (Extracellular) |
DSHB |
| Catenin complex antibodies | ||||||
| Ab6301 (anti-beta catenin) | Mouse IgG1 |
Chicken | 1:500 | Recombinant full length protein | N/A | Abcam |
| 4989S (catenin δ- 1 antibody) | Rabbit IgG |
Human | 1:200 | Synthetic peptide | N/A | Cell Signaling Technology |