Table 1.
Primer sequences used to detect genes of interest
| Gene | Primer Sequence | Amplicon Size, bp |
|---|---|---|
|
αENaC Forward Reverse |
5′‐CCCAAGGGAGTTGAGTTCTG‐3′ 5′‐AGGCGCCCTGCAGTTTAT‐3′ |
76 |
|
βENaC Forward Reverse |
5′‐GGACCAGAGCTAAATATCACC‐3′ 5′‐CGGTAGTTGAACTCTTGGAAGTAGA‐3′ |
78 |
|
γENaC Forward Reverse |
5′‐CCAGTACAGCCAGCCTCTG‐3′ 5′‐CTGGTACAACTGGTAGTAGCAATACAT‐3′ |
91 |
|
MR Forward Reverse |
5′‐CTCCCTAACATGTCCTAGAAAAGC‐3′ 5′‐AGAACGCTCCAAGGTCTGAG‐3 |
112 |
|
OT Forward Reverse |
5′‐CGCCTGCTACATCCAGAACT‐3′ 5′‐CCGCAGGGAAGACACTTG |
81 |
|
VP Forward Reverse |
5′‐TCCGACATGGAGCTGAGAC‐3′ 5′ –GGGCAGGTAGTTCTCCTCCT‐3′ |
147 |