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. 2018 Aug 28;8(9):391. doi: 10.1007/s13205-018-1416-7

Fig. 2.

Fig. 2

a Semi-quantitative RT-PCR analysis of the selected transformants; The upper panel shows the PCR product using the primers designed for the replication of the housekeeping gene, elf1α, served as the internal control, and the lower panel shows a 120 bp PCR product of CBD-alfAFP recombinant gene in selected transgenic plants; b distribution of individual transformants over different classes of transgenic mRNA expression; The panel defines four different mRNA expression levels in transformants: none (N), low (L), medium (M), and high (H)