Fig. 5.

Mean relative gene expression levels in the ARC and PVN punches for 20-week-old offspring. a mRNA expression levels of Pomc, Agrp, Npy in the ARC. b Relative mRNA levels of Ob-Rb in the ARC (two-way ANOVA: maternal diet × postweaning diet interaction, P = 0.0002; post-hoc Tukey’s test, n = 6). c Relative mRNA levels of Mc4r in the PVN analyzed by qRT-PCR in the offspring at week 20 and Npy1r (two-way ANOVA: †maternal diet effect, P = 0.0136; ‡postweaning diet effect, P = 0.0192; post-hoc Tukey’s test, **P < 0.01 compared with D-LF/O-LF, n = 6). Data are shown as mean ± SEM. *P < 0.05, **P < 0.01