Figure 2.

Inversion-type class switching in SCR2(μ, α)- (A) and SCR2(μ, Rα)- (B) derived clones. For each clone, the upper and lower sequences represent preswitch and postswitch sequences, respectively. Open arrows in the center of each sequence indicate the orientation of a segment between the two S regions. The deletion of S_μ (magenta for nontemplate strand) and S_α (green for nontemplate strand) sequence is marked by black bars on the preswitch S regions. The duplication is marked with magenta bars on preswitch and postswitch sequences. Mutations after CSR are shown in blue letters. Magenta and green arrows indicate the orientation of transcription of S_μ and S_α, respectively. The numbers on the sequences indicate positions in S regions for the beginning and end of deletions or duplications. In A, the positions of nucleotides at S_μ and S_α side border of upstream junction are indicated by a (516 for clone no. 114, see Table 1) and b (386), respectively. Similarly, positions of the downstream junction are indicated by e (530) and d (371). The lengths of deletion in S_μ and S_α are calculated by e − a − 1 and b − d − 1, respectively.