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. 2018 Aug 28;9(9):849. doi: 10.1038/s41419-018-0877-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a, b Kaplan–Meier curves and log-rank test showing the prognostic value of COL10A1 mRNA in OS and RFS in 103 gastric cancer patients. c SOX9 and COL10A1 proteins were detected in GC cell lines by western blot analysis. d SOX9 and COL10A1 were localized by confocal immunofluorescence microscopy in the SGC7901 and AGS cell lines. e, f SOX9 and COL10A1 mRNA expression levels in normal and GC tissues. The mRNA expression levels of SOX9 and COL10A1 were semi-quantified, and the correlation between these two genes was evaluated with Spearman’s rank correlation method. g Proteins isolated from normal and GC tissues were analyzed by western blot analysis of SOX9 and COL10A1. The upper panel represents Stage I normal and tumor specimens; the lower panel represents Stage IV normal and tumor specimens. h and i SOX9 and COL10A1 protein expression levels in normal and GC tissues. The protein expression levels of SOX9 and COL10A1 were semi-quantified, and the correlations between these two genes were evaluated with Spearman’s rank correlation method. Scale bars represent 10 μm in (d)