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. 2018 Aug 28;9(9):849. doi: 10.1038/s41419-018-0877-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The protein expression of COL10A1 in tumors with mLNs was determined using IHC staining. mLNs metastatic lymph nodes. b Visualization of COL10A1 (red) staining in normal and GC tissues by immunofluorescence microscopy. c COL10A1 protein expression was determined by western blot analysis after transfection of GC cell lines with COL10A1-sense plasmids and COL10A1-siRNA. d Phase-contrast microscopy visualization of the morphology of SGC7901 cells by transfection of vector and COL10A1 and MKN45 cells by transfection of COL10A1 siRNA lentivirus. e COL10A1 overexpression significantly enhanced the migration ability of cells compared with vector, while knockdown of COL10A1 achieved the opposite effect. ***P < 0.001. f Representative figures of transwell assays in SGC7901 and MKN45 cells after treatment with small interfering RNA and/or plasmid are shown. ***P < 0.001. All results shown were reproduced in at least three independent experiments. Scale bars represent 100 μm in (a, b) and 20 μm in (d)