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. 2018 Aug 28;9(9):849. doi: 10.1038/s41419-018-0877-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b EMT biomarkers (E-cadherin and Vimentin) were detected after transfection of COL10A1-sense plasmids into the indicated cells using western blot analysis and immunofluorescence. c EMT biomarkers were detected after transfection of MKN45 cells with COL10A1-siRNA using western blot analysis. d MMPs (MMP 2, 3, 9, and 14) were detected after transfection of MKN45 cells with COL10A1-siRNA using western blot analysis. e Proteins extracted from AGS cells co-treated with TGF-β1 and the inhibitor SB431542, SOX9 siRNA or COL10A1 siRNA were used to detect EMT markers, phosphorylated Smad2, SOX9 and COL10A1 using western blotting analysis. f Representative figures of transwell assays in AGS cells after treatment with small interfering RNA and/or TGF-β1 are shown. ***P < 0.001. g SOX9, COL10A1, and Ki67 expression levels in peritoneal metastatic specimens were detected using IHC. All results shown were reproduced in at least three independent experiments. Scale bars represent 60 μm in (b, g)