Fig. 2.

DVL-2 mediates non-canonical Wnt-induced ERK1/2 activation and down-regulation of COL2A1 expression. a Detection of total DVL subtypes. β-actin was used as a loading control (n = 3). b Detection of DVL-1, c DVL-2 and d DVL-3 in human primary chondrocytes with or without transfection with siRNA specific to DVL-1 (Si-DVL-1), DVL-2 (Si-DVL-2), DVL-3 (Si-DVL-3), or siRNA control (Si-control). β-Actin was used as a loading control (n = 3). e Fold changes of COL2A1 expression normalized to control in primary chondrocytes transfected with siRNA specific to DVL-1, DVL-2, DVL-3, or siRNA control and treated with Wnt-3a (n = 3). f Detection of phosphorylated and total ERK1/2 in primary chondrocytes transfected with siRNA specific to DVL-1, DVL-2, DVL-3, or siRNA control and treated or not with Wnt-3a or vehicle. β-Actin was used as a loading control (n = 3). Q-PCR values were normalized for the housekeeping gene ribosomal protein S29 and are expressed as the relative expression compared with control. One representative blot of three independent experiments is shown. Data are expressed as mean ± S.D. Statistical analysis was performed with an unpaired Student’s t test (*p < 0.05; **p < 0.01)