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. 2018 Aug 28;8:12933. doi: 10.1038/s41598-018-30979-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Deletion of rlpA partially alleviates growth inhibition of the temperature-sensitive E. coli strain LP11-1 (ftsK44). Growth curves of ΔrlpA knockout (W3110 ΔrlpA and LP11-1 ΔrlpA) and complemented (LP11-1 ΔrlpA pSG002-1) E. coli strains grown at permissive (30 °C) and nonpermissive (42 °C) temperatures (n = 3 per strain per temperature). The A₆₀₀ of two technical replicates of each culture were sampled every hour using a SmartSpec^™ Plus Spectrophotometer (Bio-Rad). In both panels, E. coli strains W3110 and LP11-1 were analyzed as WT controls. Pairwise comparison of growth differences was made using a one-way analysis of variance with Tukey-Kramer multiple comparison post-tests (*p < 0.05 versus LP11-1 at the same time point).