Table 1.
Bacterial strains and plasmids.
| Strain or plasmid | Description | Source or Reference |
|---|---|---|
| E. coli strain | ||
| W3110 | rph-1IN (rrnD-rrnE) | Coli Genetic Stock Center |
| Lemo21 (DE3) | fhuA2 [lon] ompT gal (λDE3) [dcm] ΔhsdS/ pLemo(CamR) λ DE3 = λ sBamHIo ΔEcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 Δnin5 pLemo = pACYC184-PrhaBAD-lysY | New England Biolabs |
| DH5α | F−Φ80lacZΔM15Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 | Invitrogen |
| (rK−, mK+) phoA supE44 λ-thi-1 gyrA96 relA1 | ||
| LP11-1 | W3110 ftsK44 aroA::Tn10 | K. Young69 |
| LP11-1 ΔrlpA | W3110 ftsK44 aroA::Tn10 ΔrlpA::kan | This study |
| W3110 ΔrlpA | W3110 ΔrlpA::kan | This study |
| Plasmids | ||
| pEVOL-pBpF | Expression vector encoding tRNA/aminoacyl-tRNA synthetase pair derived from Methanococcus jannaschii for in vivo incorporation of the photo-crosslinker p-benzoyl-l-phenylalanine (pBpa) into proteins in E. coli; CamR | Addgene (no. 31190)43 |
| pKD4 | Template plasmid for λ red mediated gene deletion; KanR | 68 |
| pKD46 | Red recombinase expression plasmid under the control of Para; AmpR | 68 |
| pBAD24 | Protein expression vector under the control of Para; AmpR | Addgene |
| pWQ743 | Protein expression vector under the control of Ptet; AmpR | C. Whitfield41 |
| pET28a | Protein expression vector under the control of Plac; KanR | EMD Millipore |
| pWQ572 | Protein expression vector under the control of Ptet; CamR | C. Whitfield41 |
| pAB006-2 | pBAD24 derivative encoding amino acids 1-220 of FtsK (His10-FtsKN(220)) from E. coli | 25 |
| pAB008-1 | pWQ743 derivative encoding amino acids 1-220 of FtsK (His10-FtsKN(220)) from E. coli | This study |
| pSG001-1 | pET28a derivative encoding amino acids 18-362 of RlpA with an N-terminal FLAG-tag (FLAG-RlpA) from E. coli | This study |
| pSG002-1 | pWQ572 derivative encoding amino acids 18-362 of RlpA with an N-terminal FLAG-tag (FLAG-RlpA) from E. coli | This study |
The abbreviations used are as follows: Amp, ampicillin; Cam, chloramphenicol; Kan, kanamycin.