. 2018 Aug 28;8:12933. doi: 10.1038/s41598-018-30979-5

Table 2.

Oligonucleotide pairs used for site-directed mutagenesis.

Mutation	Sequence of mutagenic oligonucleotide (5′ to 3′)^a	Plasmid
W51*	CGGACCCCAGCTAGTCGCAAACGGC	pAB008-W51*
W51*	GCCGTTTGCGACTAGCTGGGGTCCG	pAB008-W51*
D135*	CTGGCGGCAATCAACGCTTAGGATATCTGGTATTTTCGG	pAB008-D135*
D135*	GGCAAAATACCAGATATCCTAAGCGTTGATTGCCGCCAG	pAB008-D135*
D136*	GCGGCAATCAACGCTGACTAGATCTGGTATTTTGCCTCC	pAB008-D136*
D136*	GGAGGCAAAATACCAGATCTAGTCAGCGTTGATTGCCGC	pAB008-D136*
Y139*	CTGACGATATCTGGTAGTTTGCCTCCGGTGGCG	pAB008-Y139*
Y139*	CGCCACCGGAGGCAAACTACCAGATATCGTCAG	pAB008-Y139*
L158*	CACTACGCTACAACCACTGTAGCACAGTAGCGGGGGAACTA	pAB008-L158*
L158*	TAGTTCCCCCGCTACTGTGCTACAGTGGTTGTAGCGTAGTG	pAB008-L158*

^aBase changes are underlined and in boldface.