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. 2018 Aug 28;8:12947. doi: 10.1038/s41598-018-31221-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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AuNP colorimetric aggregation-based sensing method. The Figure illustrates the proposed suppression effect of nonbinding flanking nucleotide on target binding signals, adhering to the particles after target detection, which prevents aggregation, and the role of the proposed method (centrifugation and resuspension) to eliminate the residual adhesion of these nonbinding sequences.