Fig. 2.

Dsh mediates the ephrinB2–TBC1d24 interaction. a Co-IP using gastrula embryos injected with RNA encoding TBC1d24-V5 and Dsh-HA (500 pg) shows that TBC1d24 binds to Dsh. b Illustration of the deletion mutants of Dsh. Co-IP using gastrula embryos injected with RNA encoding TBC1d24-V5 and domain deletion mutants of Dsh-HA shows that the DEP domain of Dsh associates with TBC1d24. c Co-IP using gastrula embryos injected with RNA encoding Dsh-HA and wild-type TBC1d24-V5 or the mutant TBC1d24-ΔF shows that the F region is required for an interaction with Dsh. d IP using gastrula embryos injected with RNA for TBC1d24-V5 and Dsh-HA along with Dsh2 morpholino oligonucleotides (Dsh2-MO) shows that knockdown of Dsh2 suppresses the interaction between ephrinB2 and TBC1d24, and this is rescued by the expression of MO-resistant Dsh2 (Dsh2-MOR). e IF of ectoderm from the embryos injected with the indicated MOs and RNAs shows that ephrinB2 (red) induces membrane localisation of TBC1d24 (green). This localisation is dramatically reduced by knockdown of Dsh2 and also rescued by introduction of Dsh2-MOR RNA. Bar, 50 μm