Fig. 1. Knockdown of BAG3 inhibits migration and invasion of PDACs via reduction of IL-8 production.

a BAG3 was knocked down using CAS9 system, and BAG3 expression was confirmed by western blot. b–c the migration and invasion of control or BAG3 knockdown (KD) cells was evaluated by a Matrigel-uncoated (b) and coated (c) transwell assay, respectively. d Cytokine release by control or BAG3 knockdown PDACs was analyzed using cytokine antibody microarray. e–f BxPC3 cells were exposed to the indicated antibodies, cell migration and invasion was evaluated by a Matrigel-uncoated (e) and coated (f) transwell assay, respectively. g–h SW1990 cells were exposed to the indicated antibodies, cell migration, and invasion was evaluated by a Matrigel-uncoated (g) and coated (h) transwell assay, respectively. N.S., not significant; *, P < 0.01