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. 2018 Aug 28;8:12991. doi: 10.1038/s41598-018-31285-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(A) In-vitro Phantom studies: (i-ii) MRI images of phantoms of MNPs and concentration (0–100 µM) dependent T2 decrease in contrast; iii-iv) T2-decay and T1 recovery images at various concentrations of the MNP suspended in 1% agar solution in different condition i.e. 1, 5, 10, 20 and 100 uM; (B) In-vivo MRI studies: Data represented NF treated (20 mg/Kg) mice brain after 3 hrs of injection. Changes in T2 relaxation before and after intravenous administration of magneto-liposome NF: (i) T2 relaxation map at baseline, 30–90 minutes post administration; (ii) Average T2 signal intensity from muscle surrounding brain in the head (Magnet condition (0.8 T) received head magnet placement prior to imaging for 3 hours; no magnet condition were without magnet treatment); (iii-iv) T2 signal intensity from different region of brain (different color bullet represents the different location of brain as given below the image).