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. 2018 Aug 28;9(9):845. doi: 10.1038/s41419-018-0939-5

Fig. 10. Inhibited the integrin β3/FAK/PI3K/AKT signaling pathway, the role of rhMFG-E8 on neuroprotection was evaluated by Fluoro-Jade C (FJC), brain water content, and neurological severity score at 24 h after TBI.

Fig. 10

Representative immunofluorescence staining images of FJC (green). The boundary of injury cortex was labeled by the red trajectory (a). The results showed treatment of rhMFG-E8 decreased the amount of FJC-positive cells, while increased it by Integrin β3 siRNA and LY294002 (5 and 20 µM) at 24 h after TBI (b). Diagram of coronal rat brain section showing the location of lesion cavity (red) and photograph region (red squares) (c). Administration of rhMFG-E8 decreased brain edema in the ipsilateral cortex, meanwhile decreased the neurological severity score at 24 h after TBI, while integrin β3 siRNA and LY294002 (5 and 20 µM) inverted the role of rhMFG-E8 on neuroprotection following TBI (d, e). The quantitative data are the mean ± SD (n = 6 each; *P < 0.05, **P < 0.01 vs. TBI + Vehicle group; #P < 0.05, ##P < 0.01 vs. TBI + rhMFG-E8 group; nsP > 0.05). Bar = 20 µm