Fig. 8. The role of integrin β3/FAK/PI3K/AKT signaling pathway on rhMFG-E8 of anti-apoptosis was evaluated by western blotting at 24 h after TBI.

Western blotting showed that treatment with PI3K inhibitor, LY294002 (5 and 20 µM), decreased the expressions of p-AKT (a, b), high dosage exhibited better effect, while had no effect on integrin β3 and p-FAK (a, c, d). The expressions of pro-apoptosis-associated proteins, Cleaved caspase-3, and Bax were increased after administration of LY294002 (e, f, h), while the level of anti-apoptosis protein Bcl-2 was decreased (e, g). The quantitative data are the mean ± SD (n = 6 each; *P < 0.05, **P < 0.01 vs. TBI + Vehicle group; ^#P < 0.05, ^##P < 0.01 vs. TBI + rhMFG-E8 group; ^nsP > 0.05)