Fig. 9. Inhibited the integrin β3/FAK/PI3K/AKT signaling pathway, the role of rhMFG-E8 on anti-apoptosis was evaluated by immunofluorescence co-staining at 24 h after TBI.

Representative immunofluorescence co-staining images of Cleaved Caspase-3/NeuN, (Cleaved caspase-3 = red, NeuN = green, DAPI = blue) (a). The results showed treatment of rhMFG-E8 decreased the amount of Cleaved caspase-3-positive neurons, while Integrin β3 siRNA and LY294002 (5 and 20 µM) significantly reversed the role of rhMFG-E8 on anti-apoptosis at 24 h after TBI (b). Diagram of coronal rat brain section showing the location of lesion cavity (red) and photograph region (red squares) (c). Bar = 20 µm