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. 2018 Aug 28;9(9):855. doi: 10.1038/s41419-018-0900-7

Fig. 5. AF1q regulated CD44 expression in CML.

Fig. 5

a CD44 expression in K562/G01 cells transduced with AF1q siRNA or scrambled control was measured by qRT-PCR and western blot. b CD44 expression in K562 cells infected with lentiviruses harboring AF1q (K562-AF1q) or negative control (K562-NC) was measured by qRT-PCR and western blot. c CD44 expression was measured by qRT-PCR in primary BMMCs from CP CML patients (n = 5) transduced with AF1q siRNA or scrambled control. d CD44 expression in CD34+ CML cells (n = 3) transduced with AF1q siRNA or scrambled control was measured by qRT-PCR. e ChIP analysis of AF1q binding to CD44 promoter region. f Frequencies of CD44 v5, v6, v7, and v10 were measured by flow cytometry in K562 cells infected with AF1q lentiviruses. Red represents K562-NC and green represents K562-AF1q. **P < 0.01, ****P < 0.0001