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. 2001 Nov 6;98(24):13866–13871. doi: 10.1073/pnas.231486598

Figure 4.

Figure 4

Recruitment and phosphorylation of SHP-2 to tyrosine-phosphorylated FcPD. (A) IIA1.6 cells expressing FcPD, FcγRIIB, and FcKIR were stimulated, and cell lysates were immunoprecipitated (IP) by anti-flag mAb and analyzed for interaction with SHP-2 by Western blotting. (B) FcPD-expressing IIA1.6 cells were stimulated with intact Abs or F(ab′)2 fragments of anti-mouse IgG for the indicated intervals. Tyrosine phosphorylation of FcPD, association between FcPD and SHP-2, and tyrosine phosphorylation of SHP-2 were determined as described in Materials and Methods. (C) Tyrosine phosphorylation of tyrosine mutants of FcPD and association between tyrosine mutants of FcPD and SHP-2 were determined as above. Closed and open arrowheads indicate full-length and truncated FcPD chimeras, respectively.