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. 2018 Aug 28;8:12985. doi: 10.1038/s41598-018-31231-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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3D rendered membranes with and without sub-slice reconstruction. (a,b) Views of region of hepatocyte in the y-z orthoslices of Fig. 5(d,f) show clear separation of membranes after sub-slice reconstruction. (c,e) Views of three membranes indicated by arrow in (a,b) obtained from the averaged image stacks acquired at beam energies of 1.0 keV and 1.4 keV, i.e., without sub-slice reconstruction, showing that it is not possible to segment the green and red membranes accurately due to the limited z-resolution of 25 nm in the individual datasets acquired at each beam energy. (d,f) Same views as in (c,e) but after sub-slice reconstruction, again showing a clear separation of the green and red membranes. Scale bar, 500 nm.