Fig. 8. Local inhibition of ADAM17 activity in the injured cortex promotes the generation of cholinergic neurons.

Mechanical cortical lesions were unilaterally performed in the primary motor cortex of adult mice and, immediately, the lesion was locally injected with the construct expressing the Pro domain region of ADAM17 (ADAM17-Pro). Mice were then intraperitoneally injected with BrdU every 2 days during the first 14 days, and were sacrificed on day 28 post injury. a Representative images of the area surrounding cortical lesions showing proliferating BrdU⁺ cells, the cholinergic neuron marker ChAT, the GABAergic neuron marker parvalbumin, and the glutamatergic neuron marker V-Glut. Scale bar = 100 µm. b Graph shows the percentage of BrdU⁺ cells that coexpressed ChAT or Parvalbumin. Glutamatergic neurons were not found. c Representative image of merge in panel A (zoom in) left column showing double-labeled cells (BrdU⁺/ChAT⁺) in the perilesional area. Scale bar = 50 µm. Dotted lines delineate cortical lesion borders. L lesion