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. 2018 Aug 22;12:66. doi: 10.3389/fncir.2018.00066

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Copyright © 2018 Jo, Xu, Deniz, Cherian, DeVries and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The Pvalb-FlpE driver targeted multiple RGC types. (A) The Pvalb-FlpE driver was crossed with CMV-Cre and Ai65 mice. Immunostainings were performed by using antibody markers for 4 different RGC types: Osteopontin for alpha RGCs, CART for ooDSGCs, FOXP2 for F-RGCs, and melanopsin for ipRGCs. Scale bar, 20 μm. (B) Proportions of RGC types targeted in the Pvalb-FlpE driver. n = 6 retinas from 6 animals (6 litters). (C) RGC types extracted from CAGGCre-ER;Pvalb-FlpE;Ai65 retinas without tamoxifen administration. Flat-mount view (top) and side view (bottom) with ChAT (blue) labeling. Scale bar: 50 μm for the flat-mount view, 10 μm for the side view.