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. 2018 Aug 22;12:66. doi: 10.3389/fncir.2018.00066

FIGURE 4.

FIGURE 4

The Sst-FlpO driver targeted both RGCs and ACs. (A) (i) To identify targeted RGCs, the Sst-FlpO driver was crossed with Vglut2-Cre and Ai65 reporter mice. (ii) To identify targeted amacrine cells, the Sst-FlpO driver was crossed with Slc32a1-Cre and Ai65 mice. Scale bar, 50μm. (B) Staining for RGC markers (green). RGC constituents were probed with antibodies against Osteopontin, CART, FOXP2 and melanopsin in the Vglut2-Cre;Sst-FlpO;Ai65 retinas. Scale bar, 20 μm. (C) Proportions of RGC types targeted in the Sst-FlpO driver. n = 7 retinas from 7 animals (6 litters). (D) Individual RGC types extracted from the Vglut2-Cre;Sst-FlpO;Ai65 retinas. Flat-mount views (top) and side views (bottom) with ChAT (blue). Scale bar: 50 μm for the flat-mount view, 10 μm for the side view. (E–G) Amacrine cell types extracted from the UBC-CreER2;Sst-FlpO;Ai65 retinas, without tamoxifen administration. (E) Representative images of an SST-1 AC. (i) Flat-mount view of the soma and surrounding processes. (ii) Side view of the soma and surrounding “dendrite-like” and “axon-like” processes. (iii) Axon-like processes traveled across the IPL and ended at the INL border (iv). An SST-1 AC was co-labeled with antibodies against GABA (v) and SST (vi). (F) A starburst amacrine cell (SAC) in the GCL. Flat-mount view (top) and side view (bottom) with ChAT (blue). (G) SST-2 AC. Flat-mount view of the soma and surrounding processes. (ii) Side view of the soma and surrounding dendrite-like (white arrow) and axon-like processes (green arrow). (iii) Axon-like process end at the INL border. An SST-2 AC was co-labeled with antibodies against GABA (iv) and SST (v). Scale bar for (E–G): 50 μm for the flat-mount view, 10 μm for the side view.