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. 2018 Aug 22;12:66. doi: 10.3389/fncir.2018.00066

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Copyright © 2018 Jo, Xu, Deniz, Cherian, DeVries and Zhu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The Vip-FlpO driver targeted 3 types of ACs. The Vip-FlpO driver was crossed with UBC-CreER2 and Ai65, 20 μg tamoxifen was administered to achieve sparse labeling of ACs. Representative images for VIP-1 AC (A), VIP-2 AC (B), and VIP-3 AC (C) are shown in flat-mount view (top) and side view (bottom) with ChAT (blue) in (i). Arrow in (Ai) shows the “tail” of the VIP-1 AC. Sections were co-labeled with antibodies against GABA (green) (ii) and VIP (green) (iii). Scale bar: 25 μm for flat-mount view, 10 μm for side view.