Figure 2.

VPM preparation and CPD procedure preserve podosome organization. (A) DCs were seeded on glass coverslips and after VPM preparation, cells were fixed and stained for actin (cyan), vinculin (green) and zyxin (magenta). After CPD, DCs were imaged by SEM (gray). Shown are representative images of all three channels in 3 dimensions and the corresponding SEM image. Insets depict two representative podosomes within the cluster. (B) Shown are the SEM-LM overlays for all three channels for the same cells as in (A) Scale bar = 5 μm.